Microscopic examination of the DNA using the FISH method

The method of fluorescence in situ hybridization (FISH) enables the detection and characterization of numerical and structural chromosomal changes and is used in addition to conventional cytogenetics or it is used to identify the ribosomal DNA of bacteria and other pathogens. The aim of the method is to bind DNA probes, marked with a fluorescent dye, to the chromosomal DNA of the patient or the pathogen and thereby mark individual chromosome sections under the microscope in color and make any chromosome changes or chromosome building blocks visible and determine them. A fluorescence microscope, which is best suited for this purpose, is used for this examination

The goal of fluorescence in situ hybridization (FISH) is to visualize potential human chromosomal abnormalities or bacterial ribosomal DNA using DNA probes labeled with a fluorescent dye. The fluorescence microscope is the tool of choice for this.